@article{David_Fofue_Macayan_Tiongson_2017, title={Buffered Zinc Acetate Pre-treatment of Hemolyzed Serum Samples for Total Bilirubin Assay}, volume={5}, url={https://jurnal.unai.edu/index.php/isc/article/view/1574}, DOI={10.35974/isc.v5i1.1574}, abstractNote={<p><span style="font-weight: 400;">The research aimed to formulate a way of eliminating interference in total bilirubin assay due to free hemoglobin in hemolyzed serum samples. This normally precipitates hemoglobin but did not result in a clear serum. Nevertheless, the effects of this metal cation precipitation technique in the results of total bilirubin assay were compared to the results of hemolyzed and non-hemolyzed serum controls to test the hypothesis that hemoglobin can be efficiently precipitated by zinc to check for hemolysis without causing another interference. The study utilized 120 uL of 0.12 M buffered zinc acetate to precipitate 0.9 g/dL hemoglobin in 1000-uL hemolyzed serum sample. Three of fifteen sets of samples were randomly considered for testing to determine possible changes before and after pre- treatment. The result has shown that 0.12 M of zinc acetate can precipitate 0.90 g/dL free haemoglobin. However, other suspected moieties of serum constituents or the excess zinc itself causes turbidity even after centrifugation, suggesting technique modification. Therefore, further trials must be reconsidered.</span></p>}, number={1}, journal={Abstract Proceedings International Scholars Conference}, author={David, Mizael M. and Fofue, Anna Liza U. and Macayan, Yanna Yvonne C. and Tiongson, Michael Joshua F.}, year={2017}, month={Oct.}, pages={33} }