In vitro study of Angelica keiskei as Medium for Gram-Negative Bacilli Guilther Bicera, Naphi Yaen M.

Abstract

Angelica keiskei extract was used as an additive to nutrient agar in creating an alternative gram-negative selective media that may prove useful for places that has scarcity of laboratory supplies. The test used routine laboratory isolation techniques. The gram-negative bacteria tested were Escherichia coli, pseudomonas aeruginosa, and Klebsiella pneumoniae while Staphylococcus aureus and Staphylococcus epidermidis were used for the gram-positive bacteria. The result shows that both gram-positive bacteria decreased in growth proportional to the concentration of chalcone used. Both S. aureus and S. epidermidis showed no growth at 50% of chalcone concentration while all the gram-negative bacteria tested showed growth but varied through all the different concentrations tested.