Buffered Zinc Acetate Pre-treatment of Hemolyzed Serum Samples for Total Bilirubin Assay

Authors

  • Mizael M. David Adventist University of The Philippines
  • Anna Liza U. Fofue Adventist University of The Philippines
  • Yanna Yvonne C. Macayan Adventist University of The Philippines
  • Michael Joshua F. Tiongson Adventist University of The Philippines

https://doi.org/10.35974/isc.v5i1.1574

Keywords:

Buffered zinc acetate, hemolyzed serum samples, total bilirubin assay

Abstract

The research aimed to formulate a way of eliminating interference in total bilirubin assay due to free hemoglobin in hemolyzed serum samples. This normally precipitates hemoglobin but did not result in a clear serum. Nevertheless, the effects of this metal cation precipitation technique in the results of total bilirubin assay were compared to the results of hemolyzed and non-hemolyzed serum controls to test the hypothesis that hemoglobin can be efficiently precipitated by zinc to check for hemolysis without causing another interference. The study utilized 120 uL of 0.12 M buffered zinc acetate to precipitate 0.9 g/dL hemoglobin in 1000-uL hemolyzed serum sample. Three of fifteen sets of samples were randomly considered for testing to determine possible changes before and after pre- treatment. The result has shown that 0.12 M of zinc acetate can precipitate 0.90 g/dL free haemoglobin. However, other suspected moieties of serum constituents or the excess zinc itself causes turbidity even after centrifugation, suggesting technique modification. Therefore, further trials must be reconsidered.

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Published

2017-10-30

How to Cite

David, M. M., Fofue, A. L. U., Macayan, Y. Y. C., & Tiongson, M. J. F. (2017). Buffered Zinc Acetate Pre-treatment of Hemolyzed Serum Samples for Total Bilirubin Assay. 11th International Scholars Conference, 5(1), 33. https://doi.org/10.35974/isc.v5i1.1574