Hypoglycemic Activity of Extract Leaf and Root Plant of Jombang (Taraxacum Officinale) in Alloxan-Induced Diabetic Wistar Male Rats

Abstract

Diabetes mellitus (DM) is a serious health problem worldwide, World Health Organization
(WHO) declared Indonesia takes the fourth place in the DM problem. To overcome this
problem, a synthesis drug and medicines have been produced. But production from this
medicine has side effects and require a lot of money for it, while many try to find a solution
to this disease. There are plants that can help in lowering blood glucose and that is Jombang
as we known as dandelion. Because of this plant ability, this research is made. The purpose of
this study is to obtain accurate data for lowering white rat’s blood glucose from 1 g extract of
leaf and root as determined volume is 0.4 ml, 0.6 ml, and 0.8 ml from leaf extract each
contains 0.65 g, 0.97 g, and 1.29 g and root extract each contains 0.77 g, 1.15 g, and 1.54 g
weight wet of herbs. This study uses True Experimental Design, 22 male rats are divided into
five groups: Negative Control (Group I), Positive Control (group II), Experiment I (group
III): Rats were induced by alloxan and given extracted jombang leaves orally as much as 0.4
ml (0.65 gram), 0.6 ml (0.97 gram), and 0.8 ml (1.29gram), Experiment II (group IV): Rats
were induced by alloxan and given extracted jombang roots orally as much as 0.4 ml (0.77
grams), 0.6 ml (1.15 grams) and 0.8 ml (1.54 grams) and Experiment III (Group v): Rats
were induced by alloxan and given extracted as much mixture of leaves and roots of Jombang
0.4 ml (0.71 gram), 0.6 ml (1.06 gram) and 0.8 ml (1.42 gram). Treatment started from day 6
to day 15 and administered twice a day every 12-hour. The measurement of data was
collected on day 6, 9, 12, and 15. The data obtained is analyzed with logistic regression,
ANOVA multivariate followed by Duncan’s multiple range test. The result showed that the
volume of group leaf extract, robot extract and the mixture of leaf and root extract 0.4 ml

(0.65 g), 0.6 ml (0.97 g), and 0.8 ml (1.29 ml) can reducing blood glucose levels in alloxan-
induced rats at the end of the Study. The best volume of extract in lowering blood glucose is

0.4 ml (1.42 g) mixture of leaf and root extracts of Jombang.