Hemostatic Activity of Saba Banana (Musa sapientum Linn. Var. Compressa) Peel Extract on Sprague Dawley Rats

Abstract

Cardava banana (Musa sapientum Linn. var. compressa) is one of the most important banana cultivars in the Philippines. Recent studies reported that unripe banana peel contains tannins and leucocyanidin, which can accelerate and aid wound healing. The objective of the study is to evaluate the anti-hemorrhagic effect of the different concentrations of Musa sapientum Linn. var. compressa (MSVC) extract using Duke’s modified bleeding time before and after application of the MSVC extract in different concentrations; 100%, 75% and 50%. Extract of the unripe MSVC was taken from 24 kilograms dried banana peel that went through a drying process at 50 degrees Celsius and 10% relative humidity (%RH) using a Multi-Commodity Heat- Pump Dryer (MCHPD). All the rats were sedated with Zoletil 50. Rats were precisely wounded intraorally at the buccal vestibule of the lower central incisors using surgical blade number 15. The depth and length of the wounds were controlled and measured by stopping at bone depth upon incision of about 0.5 centimeters. The wounded area was pat dried every 30 seconds, then the bleeding time was recorded every after pat drying until the bleeding stops. The results were computed statistically using ANOVA and Post Hoc test which conclusively showed that 50.0% extract concentration has the most significant difference with a p-value of 0.009 as compared with other concentrations.